Sting phosphorylation site

Author: rzjo

August undefined, 2024

網頁2024年4月12日 · Yi et al. demonstrate that ER-localized JMJD8 interacts with STING to disturb the STING-TBK1 complex formation, suppresses type I IFN responses, and promotes immune evasion in breast tumor. JMJD8 knockdown improves the efficacy of chemotherapy and immune checkpoint therapy in suppressing breast tumor growth. 網頁2024年11月16日 · STING (STimulator of INterferon Genes) mediates protective cellular response to microbial infection and tissue damage, but its aberrant activation can lead to autoinflammatory diseases. Upon ligand stimulation, the endoplasmic reticulum (ER) protein STING translocates to endosomes for induction of i …

Implications of cholesterol and sphingomyelin in STING phosphorylation …

網頁PPM1A directly dephosphorylates STING and TBK1 and prevents STING aggregation. (A) STING phosphorylation induced by TBK1 was inhibited by PPM1A-WT, but not PPM1A-R174G. HEK293 cells were ... 網頁2013年10月24日 · STING also shifted in size due to alternate phosphorylation events, plausibly involving the other three serine sites that we discovered by proteomic approaches (Figure 1 H). Slightly sustained TBK1 and IRF3 phosphorylation was also observed in the absence of ULK1, perhaps indicating that the presence of STING facilitates TBK1 stability. chloroplast without grana

Phosphorylation of innate immune adaptor proteins …

網頁2024年4月7日 · During the multiple steps in the fission process, DRP1 is the most critical factor for mitochondrial division. Various post-translational modifications, such as phosphorylation, SUMOylation, and ubiquitination, control DRP1 activity. 4 Among them, phosphorylation at serine residue 616 (S616) is one of the most studied modifications … 網頁2012年3月6日 · To this end we analyzed the phosphorylation status of STING at serine 366 (S366), a site known to be phosphorylated by TBK1 and required for further activation of IRF3, and the STING-TBK1 ... 網頁Monoclonal Antibody for studying STING (Ser366) phosphate. Cited in 50 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-STING (Ser366) (D7C3S) Rabbit Monoclonal Antibody (CST #19781) is ready to ship. chloroplast was it grana

The Regulation and Double-Edged Roles of the Deubiquitinase …

Structural basis of STING binding with and phosphorylation by TBK1

網頁2024年4月6日 · b, Effects of mutations in the C53-binding site on phosphorylation of STING, TBK1 and IRF3 induced by cGAMP and C53 in cells. HEK293T cells were … 網頁2024年3月19日 · Cryo–electron microscopy reveals that cGAMP induces allosteric conformational changes in STING to form tetramers. While apo-STING can bind to … gratuity\\u0027s 7p網頁2024年5月6日 · As for STING, TRIM38 mediates the SUMOylation of STING at K338 to inhibit STING degradation, promoting oligomerization of STING and recruitment of IRF3 (). On the other hand, at the late stage of infection, STING is deSUMOylated by SUMO-specific protease (SENP)2 after phosphorylation at S366, eventually leading to STING … gratuity\\u0027s 7o

"網頁2024年7月29日 · This review will summarize recent evidences in the field of STING-mediated cell death (including apoptosis and necrosis) and discuss relevant clinical significance. 2. Overview of STING in Apoptosis. The interaction between STING signaling and apoptosis was firstly and independently reported by White et al. [ 12] and Rongvaux … " - Sting phosphorylation site

Sting phosphorylation site

PPM1A Regulates Antiviral Signaling by Antagonizing TBK1 …

網頁2024年1月23日 · S358, Ser366, and Y245 are three phosphorylation sites for STING activation (Table 1) [25, 26]. Conversely, autophagy-related gene (ULK1) induces S366 phosphorylation, contributing to STING degradation and abolishment of IFN and inflammatory cytokines production [ 27 ]. 網頁2024年12月19日 · Poly(dA:dT) and cGAMP induce STING-dependent autophagy and degradation of STING. a Immunoblot analysis of LC3-ΙΙ expression levels in Sting wild-type and knockout MEF cells stimulated by poly(dA:dT) at a final concentration of 1 µg/ml. Quantification of LC3-II expression levels in a is shown in the right panel (mean ± s.d.; …

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網頁2024年3月6日 · Moreover, although activated TBK1 phosphorylates STING, the structural information indicates that the phosphorylation site on STING is probably located … 網頁2024年2月4日 · The phosphorylation sites of the cGAS N terminus are surrounded by diverse sequences, which are likely recognized by more than a single kinase. In our search for the kinases that phosphorylate cGAS during mitosis, we noticed that the kinetics of cGAS modification strongly correlated with histone H3S10 phosphorylation, a well …

網頁2024年8月16日 · DNA virus infection leads to quick ubiquitination and phosphorylation of STING, which is required for its trafficking and subsequent degradation in the perinuclear lysosomal compartment [19, 20]. However, RNA virus infection does not cause any post-translational modifications nor the degradation of STING [ 11 , 20 ]. 網頁2024年9月28日 · Serine phosphorylation events are also essential for both STING and IRF3 activation. The phosphorylation of STING S358 allows TBK1 to bind STING. Then, TBK1 trans-phosphorylates S366 of the adjacent STING complex, allowing the recruitment29,30,31]. ...

網頁2024年4月8日 · Activated STING triggers the phosphorylation of interferon regulator factor 3 (IRF3), which then translocases to the nucleus as a transcription factor to induce the production of type-I IFN and NF-κB for activation [26]. 網頁2024年5月14日 · Succinate treatment enhanced HIF-1α stabilization in infected STING KO macrophages (), supporting the link between HIF-1α and succinate accumulation promoted by STING. All together, these data suggest that HIF-1α stabilization by increased succinate levels is one possible mechanism underlying the contribution of macrophage metabolic …

網頁2024年3月1日 · In this binding mode, the phosphorylation site Ser366 in the STING tail cannot reach the kinase-domain active site of bound TBK1, which suggests that STING phosphorylation by TBK1 requires the ...

網頁STING蛋白是一种跨膜蛋白，通常位于内质网上，是调节固有免疫的关键因子。. Shang和Zhang等人日前在《Nature》上报道了STING的全长结构，包括STING与激酶蛋白TBK1的复合物，在STING激活时该复合物启动下游信号通路。. 细胞质中双链DNA的异常存在是激活STING的有效危险 ... chloroplast with scale bars網頁2024年3月6日 · The C-terminal tail (residue 337 to the C terminus)—which contains the phosphorylation site for TANK-binding kinase 1 (TBK1) ... Phosphorylation of STING, … gratuity\u0027s 7n網頁2024年2月4日 · STING is an intracellular sensor of pathogen- or host-derived DNA. In this study, the authors identify an ESCRT complex that regulates STING degradation, thus … chloroplast wound treatment網頁Our previous study (9) revealed that the Y245 phosphorylation of STING is a marker for late endosome trafficking and allows STING to form a tripartite complex with TBK1 and IRF3. We now find that cGAMP induces a higher level of Y245 phosphorylation in cells expressing WT or T404A-STAT2 than in cells expressing the T404E mutant ( SI Appendix … chloroplast work網頁2024年3月16日 · We observed agonist-, TBK1-, “ER-to-Golgi” traffic-, and palmitoylation-dependent phosphorylation of STING at Ser365, mirroring the nature of STING … gratuity\\u0027s 7s網頁2024年7月11日 · Primer. cGAS–STING signaling. Cyclic GMP–AMP synthase (cGAS) and stimulator of interferon genes (STING, also known as TMEM173) constitute the major signaling pathway in vertebrates that senses non-self DNA and elicits potent immune responses. At the core of this pathway, cGAS senses double-stranded DNA (dsDNA) and … gratuity\\u0027s 7r網頁2024年2月14日 · In the KRAS/TP53-mutant LUAD cell line, Calu6, we observed a significant up-regulation of multiple STING pathway proteins after cisplatin treatment, including total STING (fold change [FC] = 2.34, p < 0.001), phospho–TANK-binding kinase … chloroplasty co to