WebFeb 6, 2024 · The type III secretion system (T3SS) directly injects effector proteins into host cells, interfering with cell function or causing cell death (7, 8). ... The ITC assay was performed using a MicroCal iTC200 (Malvern) as previously described . The ligand palmitoyl-CoA (0.2 mM) was injected into the PvrA protein or the point mutants (0.02 mM) … WebWe have implemented a new pricing system. This means you will see your customer specific price when you add an item to your basket Refine Results . Categories . Consumables. Cells, Cuvettes and Cups ... MicroCal ITC200; MicroCal Auto-ITC200; MicroCal PEAQ-ITC; MicroCal PEAQ-ITC Automated; MicroCal VP-ITC; About
BCF iTC200 - 中央研究院
WebMar 21, 2024 · The MicroCal iTC200 is designed for ease-of-use and exceptional sensitivity. Its wide affinity range enables analysis of weak to high affinity binders with exceptional reproducibility.... WebMicroCal iTC200 The MicroCal iTC200 is a highly sensitive, low volume isothermal titration calorimeter for the label-free in solution study of biomolecular interactions. It delivers direct measurement of all binding parameters in a single experiment and can analyze weak to high affinity binders, using as little as 10 µg sample. Semi-automated maintenance minimizes … baut segi lima
Investigations of Lipid Binding to Acyl-CoA-Binding Proteins …
WebMar 4, 2015 · Malvern Panalytical MicroCal ITC system. ... MicroCal iTC200 is developed for high sensitivity and ease-of-use. Cell injection, filling and cell cleaning functions are semi- automated and need the least amount of operator involvement. MicroCal VP-ITC is developed for ease-of-use, providing fast, accurate analysis and very good data sensitivity ... WebJan 1, 2024 · ITC was performed at 25°C using an iTC200 System (MicroCal, GE Healthcare). WT p38α or mutant proteins were dialyzed against the titration buffer (50 mM Tris [pH 7.0], 50 mM NaCl, and 1 mM DTT). MKK6 and GRA24 KIM peptides were also prepared in the same buffer. A 70 μM protein solution was titrated using 26 stepwise … WebITC measurements were performed to measure the binding of D4 with amorphous and crystalline AH nps as well as AH gel at 25°C on a MicroCal iTC200 (Malvern Instruments Ltd, UK). All proteins and adjuvants were dissolved in … tio do tanjiro