Determination of trilostane by hplc
WebTrilostane inhibits synthesis of cortisol in dogs. It is a competitive inhibitor of 3-beta-hydroxysteroid dehydrogenate, which will interfere with the steps that lead to cortisol … WebObjective: To determine whether trilostane or ketotrilostane is more potent in dogs and determine the trilostane and ketotrilostane concentrations that inhibit adrenal gland cortisol, corticosterone, and aldosterone secretion by 50%. Sample: 24 adrenal glands from 18 mixed-breed dogs. Procedures: Adrenal gland tissues were sliced, placed in tissue …
Determination of trilostane by hplc
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WebOct 17, 2016 · The method was validated and all the factors influencing the separation and analysis were checked experimentally. The method ensures the separation of the five above-mentioned compounds with good resolutions in a relatively short run time. Accordingly, the proposed HPLC method can be applied for in-process and finished … WebDec 9, 2015 · Box-Behnken design was applied to optimize high performance liquid chromatography (HPLC) conditions for the simultaneous determination of potassium sorbate, sodium benzoate, carmoisine, allura red, ponceau 4R, and caffeine in commercial soft drinks. The experimental variables chosen were pH (6.0–7.0), flow rate (1.0–1.4 …
WebJan 2, 2024 · For HPLC analysis, after grounding, the sample of tea, granule or extract was first dissolved in methanol to make a solution at the concentration of 25 mg/mL, sonicated for 20 min. and then centrifuged. Syrup was cleaned up using a Sep-Pak cartridge. Supernatant was collected and an aliquot was injected into HPLC. WebAug 15, 2024 · The determination of caffeine content in coffee products is routinely done by RPLC in commercial applications in order to guarantee purity and quality of ground …
WebA high-performance liquid chromatographic (HPLC) method for the determination of triclosan and triclocarban in cosmetic products was developed. Triclosan and triclocarban quantities in 168 of cosmetics were investigated and statistical analyzed with this method. The optimal condition are as follows: An Agilent SB-C8 analytical column (250 × 4. ... WebJun 1, 2002 · Abstract and Figures. An isocratic reversed-phase liquid chromatographic (HPLC) method is proposed for the practical and reliable determination of triclosan, an antimicrobic agent incorporated ...
WebHigh pressure liquid chromatographic (HPLC) analysis of plasma taken over 8 h from ten normal male subjects medicated with 120 mg of trilostane revealed that the drug is rapidly metabolised into at least one metabolite, 17-keto trilostane. Both compounds were detected in the blood stream at concentr …
WebFeb 27, 2015 · A high-performance liquid chromatography–fluorescence detection (HPLC-FLD) method was developed and validated for choline quantification in foods. Samples were extracted by homogenizing in chloroform/methanol/water and hydrolyzing in HCl-acetonitrile. Choline was derivatized using 1-naphthyl isocyanate and quantified by HPLC … cycloplegic mechanism of actionWebJun 14, 1991 · A simple high-performance liquid chromatographic (HPLC) method for assaying trilostane, a synthetic steroid, and one of its metabolites, ketotrilostane, in small volumes of rat plasma has been developed. A single liquid-liquid extraction was used to … cyclophyllidean tapewormsWebThus, plasma analysis is required to support in vivo studies in rats designed to maximise absorption. Two high-performance liquid chromatographic (HPLC) assays for trilostane … cycloplegic refraction slideshareWebTrilostane ((4α,5α,17β)-3,17-dihydroxy-4,5-epoxyandrost-2-ene-2-carbonitrile); >= 98% HPLC; Trilostane has been used to evaluate its capability to regulate the sex-dependent differences in the lipopolysaccharide (LPS)-induced inflammatory responses of astrocytes; Trilostane inhibits the production cyclophyllum coprosmoidescyclopiteWebJan 1, 1984 · For quantitative analysis and for monitoring separations the mobile phase consisted of methanol--O.1 M formic acid (1:1) at a flow-rate of 2 ml/min. Both column … cyclop junctionsWebA trilostane assay was developed using reagents and chemicals of high-performance liquid chromatography (HPLC) analytical grade. An Agilent 1260 Infinity HPLC system (Santa Clara, California) equipped with a pump (model G7111A), an auto sampler (model G7129A), Kinetex C18 (150 4.6 mm, 5 μm particle size) column (Torrance, Cali- cycloplegic mydriatics