Cst atf4 antibody

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August undefined, 2024

WebThis antibody has been shown to work in applications such as: Precipitation, Sequencing, ChIP-Seq, ChIP, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot, and Other. ATF-4 (D4B8) Rabbit … WebActivating transcription factor 4 (ATF4), also known as cAMP-response element-binding protein 2 (CREB2), is a substrate of RSK2 and a basic leucine-zipper transcription factor …

Activating mutations in EGFR and PI3K promote ATF4 induction …

WebSep 5, 2024 · ATF4 is induced in CTLs exposed to tumor-derived factors and antigen stimulation. We sought to determine the expression of ATF4 in CD8 + T cells upon their activation or exposure to the stress stimuli and tumor-derived factors. Treatment of mouse splenic CD8 + T cells with thapsigargin, an inducer of the endoplasmic reticulum stress … WebMonoclonal Antibody for studying ATF-4. Cited in 661 publications. Validated for WB, IP, IF, ChIP, ChIP, C&R. Highly specific and rigorously … burlington county election results 2021

ATF4 Polyclonal Antibody (10835-1-AP) - Thermo Fisher Scientific

WebDec 17, 2024 · BackgroundThe role of activating transcription factor 4 (ATF4) underlying gastric cancer (GC) remains unclear. The purpose of this study was to investigate the expression levels and biological functions of ATF4 in GC.MethodsExpression of ATF4 was detected by quantitative PCR (qPCR), Western blotting, and immunohistochemistry. Cox … WebMay 5, 2024 · About 500 mg protein of total cell lysates was incubated with primary antibody anti-ATF4 (#11815, CST or 10835-1-ap, Proteintech group, USA) or anti-CTCF (#3418, CST or sc271514, Santa Cruz Biotechnology, USA) at 4 °C with overnight shaking. The immune complexes were then incubated with Protein A/G Dynabeads (Thermo … WebJan 10, 2024 · Moreover, ATF4 overexpression inhibited tumor proliferation and AR expression both in vitro and in vivo. Collectively, these results demonstrated that ER … burlington county elections 2021

Anti-ATF4 Antibody [S360A-24] Monoclonal IgG2a

ATF4 antibody (10835-1-AP) Proteintech - ptglab

WebOct 22, 2010 · ATF4 is a transcriptional activator of the integrated stress response, a program of gene expression involved in metabolism, nutrient uptake, anti-oxidation, and … WebNov 1, 2024 · Membranes were then incubated at 4 °C overnight with ATF4 antibody (BioLegend) at 1:1000. After washing with 1x TBST, anti-rat HRP secondary antibody (CST) was used at 1:2000. A list of commercially available antibodies tested in livers and cell lines is included in Supplementary Table 1. 2.6. Gene expression analysis. burlington county election results 2022WebCST4. Cystatin-S is a protein that in humans is encoded by the CST4 gene. [3] [4] The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. … halo top hq

"WebMar 3, 2024 · In the nervous system, activating transcription factor 4 (ATF4) is involved in the regulation of synaptic plasticity and memory formation. Here, we show that ATF4 … " - Cst atf4 antibody

Cst atf4 antibody

ATF4/ATG5 Signaling in Hypothalamic Proopiomelanocortin Neurons ...

WebATF4 Rabbit pAb (A0201) Western blot analysis of extracts of HeLa cells, using ATF4 antibody (A0201) at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 μg/mL) for 8 hours. Secondary antibody: HRP Goat Anti … WebActivating transcription factor 4 antibody; ATF 4 antibody; ATF4 antibody; ATF4 protein antibody; ATF4_HUMAN antibody; cAMP-dependent transcription factor ATF-4 antibody; cAMP-responsive element-binding …

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WebAug 13, 2024 · This was especially relevant as we found in the public CHIP-seq data base (chip-atlas.org), that the Nrf2 antibody bound to the ATF4 promoter (~1 kB from the TSS) in the cardiovascular HAEC cell ... WebMar 25, 2024 · Some oncoproteins along with stress kinase general control non-derepressible 2 (GCN2) can ensure the induction of activating transcription factor 4 (ATF4) to counteract amino acid deprivation; however, little is known regarding the role of the oncogenic EGFR-PI3K pathway. In this study, we demonstrate that both mutated EGFR …

WebAnti-ATF4 antibodies are offered by a number of suppliers. This target gene encodes the protein 'activating transcription factor 4' in humans and may also be known as CREB-2, …

WebDec 25, 2024 · The membrane was blocked and then incubated overnight at 4°C with primary antibody as described previously ... antibody to phospho-T389 p70S6K1 was obtained from Cell Signaling Technology (catalog number: 9205); antibodies to REDD1 (catalog number: 10638-1-AP), Sestrin1 (catalog number: 21668-1-AP), and Sestrin2 … WebThis antibody is a rabbit polyclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 38 kDa ATF4 protein and its phosphorylated …

Webab31390 has been referenced in 9 publications. Liu Y Hydrogen peroxide induces nucleus pulposus cell apoptosis by ATF4/CHOP signaling pathway. Exp Ther Med 20:3244-3252 (2024). PubMed: 32855694; Liu P et al. …

WebThe antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these … burlington county esuWebJun 24, 2024 · PERK IPs were performed with 1 mg of lysate incubated with 2 µg of primary antibody (Proteintech, #20582-1AP) or the corresponding IgG (Cell Signaling Technology, #2729) overnight at 4 °C with ... burlington county elderly servicesWeb1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATF4 (60035-1-Ig, Clone:3E4C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at dilution 1:1000 (red), or 0.4 ug Control … burlington county employee portal loginWeb(11815) ATF-4 (D4B8) Rabbit mAb - Cell Signaling Technology - CiteAb. 670 citations – A rabbit monoclonal antibody, raised against Cyclic AMP-dependent transcription factor ATF-4 (Human), supplied by Cell Signaling Technology. ... ATF4, Activating transcription factor 4, CREB-2, CREB2, Cyclic AMP-dependent transcription factor ATF-4, Cyclic ... burlington county employment opportunitiesWebImmunoprecipitation of ATF-4 from extracts of 293 cells, treated with tunicamycin (2 μg/ml, 8 hr), using Rabbit (DA1E) mAb IgG XP ® Isotype … halo top iceWebrepresentative of CST, are rejected and are of no force or effect. P roduc t s are labeled wit h For Res earc h Us e O nly or a s imilar labeling s t at ement and hav e not been approv ed, c leared, or lic ens ed by t he FDA or ot her regulat ory f oreign or domes t … burlington county emergency dispatchWebATF4 Antibody - Mouse monoclonal antibody to human Activating Transcription Factor 4, clone S360A-24. Validated for WB, IHC, ICC/IF with human, rat tissue. ... Cell Signaling, Epigenetics and Nuclear Signaling, Neuroscience : Cellular Localization : Cell membrane, Cytoplasm, Nucleus : Accession Number : NP_001666.2 ... burlington county ems training