Clonogenic death

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August undefined, 2024

WebMar 1, 2014 · The present study was conducted in order to evaluate the effect of Brazilian propolis (AF-08; 5, 10, 15, 30, 50, 100, and 200μg/mL) in protecting CHO-K1 cells against genotoxic and cytotoxic damage and clonogenic death induced by (60)Co gamma-radiation (1.0, 2.0, 4.0, and 6.0Gy). For this purpose, t … WebDespite inhibition of radiation-induced apoptosis by 300 micrograms/ml suramin (from 5% to 2.9% at 48 h), clonogenic cell death was enhanced by the combination of suramin and …

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WebMechanistically, Dex increased superoxide and hydrogen peroxide production and augmented radiation-induced oxidative stress and cell death of myeloma cells. In contrast, Dex inhibited radiation-induced increase in pro-oxidant levels and enhanced the clonogenic survival in normal hematopoietic stem and progenitor cells. lose flabby skin after weight loss

Clonogenic assay of cells in vitro Nature Protocols

WebDec 1, 2014 · The accretion of AVO in surviving progenies of irradiated cells, and the increased incidence of clonogenic death after inhibition of vacuolar H+-ATPase suggest that formation of acidic organelles represents a novel defense mechanism against radiation damage. Expand. 1,009. PDF. View 2 excerpts, references background and methods ... WebOct 25, 2013 · Our results also show that while in MCF-7 cells rapamycin induces clonogenic death with an IC 50 of ~50 nM, in the MDA-MB-231 cells concentrations up to 2 µM did not significantly affect cell survival (Table S2 in File S1). eIF2α is a downstream target of rapamycin and irradiation. WebMar 31, 2024 · Mitotic catastrophe (MC) is a cell death modality induced by DNA damage that involves the activation of cell cycle checkpoints such as the “DNA structure checkpoint” and “spindle assembly checkpoint” (SAC) leading to aberrant mitosis. Depending on the signal, MC can drive the cell to death or to senescence. The suppression of MC favors ... horley green road

Full article: Chitosan gold nanoparticles induce cell death in HeLa …

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WebFeb 28, 2024 · Citation 44 Thus, our results not only indicate that CH-AuNPs compromise HeLa and MCF-7 cells to clonogenic death, but also that CH-AuNPs could potentially be antimetastatic. However, more studies should be done, as it has also been reported that clonogenic analyses in some cases are not related to the metastatic potential of the cells. WebOct 25, 2013 · Our results also show that while in MCF-7 cells rapamycin induces clonogenic death with an IC 50 of ~50 nM, in the MDA-MB-231 cells concentrations up … horley harriersWebDec 27, 2024 · In plasma treatment, the clonogenic cell death was better predicted by intracellular ROS production rather than by medium OH production. Introduction Plasma is a fully or partially ionized gas medium. horley green house hx3 6as

"WebJan 27, 2024 · C Clonogenic assay was conducted in MCF-7 SELENOF cells treated twice weekly -/+ Dox for 2 weeks. Dissociated single cells were seeded at clonogenic density. Dissociated single cells were seeded ... " - Clonogenic death

Clonogenic death

Clonogenic assay of cells in vitro Nature Protocols

WebJul 18, 2024 · Clonogenic death is defined as the loss of ability of a cancer cell to proliferate indefinitely, which can only be assessed accurately by clonogenic assays … WebC. N. Apoptosis and Clonogenic Cell Death in PC3 Human Prostate Cancer Cells after Treatment with Gamma Radiation and Suramin. Radiat. Res. 148, 105-114 (1997). …

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WebJan 16, 2013 · We find that apoptosis and clonogenic cell death by genotoxicants in p53-null background is enhanced by Cry mutation exclusively for UV mimetics–induced DNA damage and no other. Furthermore, we present evidence indicating that the enhanced apoptosis and clonogenic death by UV mimetic agents in Cry-mutant background is … A clonogenic assay is a cell biology technique for studying the effectiveness of specific agents on the survival and proliferation of cells. It is frequently used in cancer research laboratories to determine the effect of drugs or radiation on proliferating tumor cells as well as for titration of Cell-killing … See more The experiment involves three major steps: 1. The treatment is applied to a sample of cells. 2. The cells are "plated" in a tissue culture vessel and allowed to grow. See more The treatment is usually a drug, ionizing radiation, or a combination of the two. Some current research studies the potentiation of drug … See more • Cancer • Microbiology • Oncology • Radiation Oncology See more

WebMar 1, 2007 · Conversely, inheritance of wild-type p53 by prostate cancer cells lacking a functional allele of this gene (i.e., DU145) significantly increases clonogenic death through p53-dependent cellular senescence and apoptotic pathways. Our data provide evidence that mutations of even one p53 allele may be sufficient to alter their clonogenic fate. WebOct 5, 2024 · Clonogenic survival was around 10–15% higher in cells lacking Bid compared with controls after TRAIL exposure , suggesting that mitochondrial signaling further promotes clonogenic death.

WebRadiation-induced cell death occurs via two main pathways, that is interphase death and clonogenic inactivation. While the former does not allow the cell to reach the first post … WebMay 1, 2000 · The data show that for this human epithelial cell line, doses of 0.01–0.5 Gy show clonogenic death by the bystander effect only, if maximum potential bystander killing is assumed. The magnitude of the effect is relatively constant, and it appears to saturate at doses in the range of 0.03–0.05 Gy. After doses greater than 0.5 Gy, the curves ...

WebThe data show that for this human epithelial cell line, doses of 0.01-0. 5 Gy show clonogenic death by the bystander effect only, if maximum potential bystander killing is …

WebClonogenic cell death. The term Clonogenic cell death does not refer to a specific mode of cell death but usually simply reflects the number of viable versus dead cells as … horley green road halifaxWebPurpose: We evaluated apoptotic, necrotic and clonogenic cell death and inhibition of cell growth in a human melanoma cell line (Sk-Mel-3) and a normal human fibroblast cell line … lose hair after pregnancyWebApr 12, 2012 · Clonogenic death of FSaII and MCF-7 cells treated with metformin alone or with radiation. (A, B) Cells were incubated for 1–48 h with 0.5–10 mM metformin and the clonogenic survival was ... horley greyhound trustWebSep 16, 2024 · Head and neck squamous cell carcinoma (HNSCC) is a challenging cancer with little change in five-year overall survival rate of 50–60% over the last two decades. Radiation with or without platinum-based drugs remains the standard of care despite limited benefit and high toxicity. HNSCCs often overexpress epidermal growth factor receptor … lose forearm fatWebDespite inhibition of radiation- induced apoptosis by 300 μg/ml suramin (from 5% to 2.9% at 48 h), clonogenic cell death was enhanced by the combination of suramin and … lose fur crosswordWebJan 1, 2014 · Definition of Cell Death. In radiobiology we usually refer to reproductive cell death. Death is defined as loss of reproductive (“clonogenic”, “colony forming”) … lose forty pounds in two monthsWebA clonogenic assay, also known as a colony formation assay. is an in vitro cell survival assay. It assesses the ability of single cells to survive and reproduce to form colonies1. This assay was first described in the 1950s, … horley gym